| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -EGFR Rabbit pAb | BKM-00143 | 100 μL |
Product Information
Description | EGFR rabbit polyclonal antibody |
Protein full name | Epidermal growth factor receptor |
Synonyms | EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse EGFR |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 134 kDa |
Observed MW. | 175 kDa |
Uniprot ID | Q01279, P00533 |
Applications
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 600-1: 1000 | HepG2, A549, A431, Hacat, Hct116 |
Background
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules.
Images
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Western blot analysis of EGFR (GB11084-1) at dilution of 1: 600 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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