| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti- HARS2 Rabbit pAb | BKM-02793 | 100 μL |
Product Information
Description | HARS2 rabbit polyclonal antibody |
Protein full name | Histidine--tRNA ligase, mitochondrial |
Synonyms | HARS2, HARSL, HARSR, HisRS, Histidine tRNA ligase, Histidine tRNA ligase like, HO3 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse HARS2 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 57 kDa |
Observed MW. | 50 kDa |
Uniprot ID | Q99KK9 |
Applications
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | kidney, uterine |
Background
HARS2, also named as HARSL, HARSR, HO3 and HisRS, belongs to the class-II aminoacyl-tRNA synthetase family. It is an enzyme that catalyzes the esterification of histidine to its cognate tRNA as an early step in protein biosynthesis. When overexpressed in HIV-1-producing cells, HARS2 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of matrix(MA) protein . HO3, through its recognition of MA, plays a role in the life cycle of HIV-1. (PMID: 9445076 ,7755634 ). 1-33aa of HARS2 is transit peptide and this part will be cut in some form(~52kd) of HARS2. This antibody can bind both HARSs for the close sequences.
Images
 | Western blot analysis of HARS2 (GB113986) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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