| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -CD63 Rabbit pAb | BKM-04318 | 50 μL |
Product Information
Description | CD63 rabbit polyclonal |
Protein full name | CD63 antigen |
Synonyms | CD63, LAMP-3, ME491, MLA1, OMA81H, TSPAN30, CD63 molecule, Lysosomal associated membrane protein 3, LAMP3, CD63 antigen, NGA, Tetraspanin-30 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse CD63 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Secreted, Cell membrane, Cytoplasm |
Uniprot ID |
Applications
applications | species | dilution | positive tissue |
IHC | Mouse, Rat | 1: 500-1: 3000 | lung, liver |
IF | Mouse, Rat | 1: 500-1: 1500 | lung, brain |
Background
CD63 antigen is a protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is a cell surface glycoprotein that is known to complex with integrins.CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak Syndrome. Also this gene CD63 has been associated with tumor progression.
Images
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IHC analysis of CD63 (GB11620). Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
|
IHC analysis of CD63 (GB11620). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of CD63 (GB11620). Sample: Rat liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
|
IHC analysis of CD63 (GB11620). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
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IF analysis of CD63 (GB11620). Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of CD63 (GB11620). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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