| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti - SARS-CoV-2 spike glycoprotein Mouse mAb | BKM-04853 | 100 μL |
Product Information
Protein full name | SARS-COV-2 S protein S1 subunit RBD |
Synonyms | SARS-COV-2 S protein S1, RBD,SARS-CoV-2 spike glycoprotein,SARS-CoV-2 S protein RBD, Covid-19 |
Immunogen | SARS-COV-2 S protein S1 subunit RBD domain recombinant protein expressed in 293F cells. |
Isotype | IgG1,κ |
Purity | Affinity purification |
Predicted MW. / Observed MW. | 25 kDa / 25 kDa/35 kDa (glycosylation) |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | ----------- | 1: 500-1: 1000 | Use at an assay dependent sample |
Elisa | ----------- | 1: 500-1: 20000 | ----------- |
Background
SARS-COV-2 S protein S1 is a powerful weapon of coronavirus SARS-COV-2 to infect human normal cell. Because the receptor binding domain (RBD) lies in S spike protein S1 subunit, which exclusively binds to human ACES2 protein, followed by SARS-COV-2 nuclear materials entering into human cells cytoplasm and starting replication.
Images
| Western blot analysis of SARS-COV-2 S protein S1 (GB12869) at dilution of 1:600. Lane 1-3: recombination RBD protein expressed in 293F cells |
| Binding Activity of GB12869 with SARS-COV-2 S protein S1. Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-COV-2 S protein S1 at 1 μg/ml can bind GB12869. The EC50 of GB12869 is 7.67 ng/ml. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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