| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Recombinant Anti-EpCAM antibody (Mouse mAb ) | BKM-05092 | 50 μL |
Product Information
Protein full name | Epithelial cell adhesion molecule |
Synonyms | EPCAM, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, epithelial cell adhesion molecule, CD326, cd326 |
Immunogen | Recombinant protein corresponding to Mouse EpCAM |
Uniprot ID | P16422 |
Isotype | IgG1 |
Purity | Affinity purification |
Subcellular location | Cell membrane |
Predicted MW. / Observed MW. | 35 kDa / 35-40 kDa |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human | 1: 500-1: 1000 | HT29, MCF7, A431 |
IHC/IF | Human | 1: 1000-1: 2000 | breast cancer, colon cancer |
Background
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Images
| WB analysis of EpCAM(GB15274). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of EpCAM(GB15274). Sample: Human breast cancer,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of EpCAM(GB15274). Sample: Human colon cancer,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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