Gene Name Synonym

FNDC5, FRCP2, irisin, Irisin, fibronectin type III domain containing 5

Species

Human

Accession

Q8NAU1

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Asp32-Thr152 is expressed with a 10×His tag at the C terminus

Molecular Weight

13.7 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. Irisin is a cleaved version of FNDC5, named after the Greek messenger goddess Iris. Fibronectin domain-containing protein 5 is a membrane protein comprising a short cytoplasmic domain, a transmembrane segment, and an ectodomain consisting of a ~100 kDa fibronectin type III (FNIII) domain.Irisin, which was recently identified as a myokine and an adipokine, transforms white adipose tissue to brown adipose tissue.Irisin can potentially prevent obesity and associated type 2 diabetes by stimulating expression of WAT browning-specific genes via the p38 MAPK and ERK pathways.In addition to the proposed effects of irisin on adipose tissue, bone, and brain described in more detail below, irisin had anti-inflammatory and antimetastatic effects, improved glucose uptake in muscle cells, facilitated endothelial cell proliferation, lowered blood pressure, and improved cardiac hypertrophy.

Data

SDS-PAGE

Bioactivity

Recombinant Human FNDC5 Protein was determined by SDS-PAGE with Coomassie Blue.

Active recombinant FNDC5 upregulated both UCP1 expression and white to brown fat conversion in mature 3T3-L1 adipocytes ( Immunofluorescence and Bodipy staining, Blue: DAPI; Green: Bodipy stain for Lipids; Red: Brown adipose tissue marker UCP1，cat: GB112174).