Gene Name Synonym

CD206, MRC1, CLEC13D, CLEC13DL, MRC1L1

Species

Human

Accession

P22897

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Leu19-Ala1389 is expressed with a 6×His tag at the C terminus

Molecular Weight

158.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD206 is expressed on M2 alternatively activated tissue macrophages including tumorassociated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells. CD206 is a conserved checkpoint mediator protein that transduces the replication stress signal to the downstream effector kinase. CD206 promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases. It is involved in T cell polarization and production of pro-and anti-inflammatory cytokines. It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which may be blocked by engagement of CD206 on TAMs by tumor mucins. The loss of mrc1 checkpoint activity results in the aberrant activation of late/dormant origins in the presence of hydroxyurea. TAMs expressing the multi-ligand endocytic receptor mannose receptor (CD206/MRC1) contribute to tumor immunosuppression, angiogenesis, metastasis, and relapse.

Data

SDS-PAGE

Bioactivity

Testing in progress.

Recombinant Human CD206 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 140-160 kDa.