| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -mTOR Rabbit pAb | BKM-00898 | 100 μL |
Product Information
Protein full name | Serine/threonine-protein kinase mTOR |
Synonyms | FK506-binding protein 12-rapamycin complex-associated protein 1, Mammalian target of rapamycin, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, mTOR |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human mTOR |
Uniprot ID | P42345 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 289 kDa / 289 kDa |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, liver, brain |
IHC/IF | Mouse, Rat | 1: 200-1: 600 | brain |
Background
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications.
Images
| WB analysis of mTOR (GB111839) |
| IHC analysis of mTOR (GB111839) Sample: Mouse brain 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of mTOR (GB111839) Sample: Rat brain 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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