Synonym

IL-1 alpha, Il1a, Hematopoietin-1, LEM, LAF, Pro interleukin 1 alpha, IL1F1, Preinterleukin 1 alpha

Species

Human

Accession

P01583

Source

CHO cells

SPECIFICATIONS

Amino acid sequence

Ser113-Ala271 is expressed with a 10×His tag at the C terminus

Molecular Weight

19.4 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

IL-1 alpha is a member of the interleukin 1 cytokine family. Located in the IL1 cluster, the IL1A gene is a product of IL1B gene duplication, and consequently IL-1α and IL-1β share a receptor, IL-1R1, and its downstream signaling. IL-1α has additional functions, represented by the presence of a nuclear localization signal within its pro-domain. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1α is a substrate of noncanonical inflammatory caspases. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human IL-1 alpha Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 μg/mL.