Synonym

IL-22, IL22, IL-TIF, ZCYTO18

Species

Mouse

Accession

Q9JJY9

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Leu34-Val179 is expressed with a 10×His tag at the C terminus

Molecular Weight

18.5 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

IL-22 is a relatively new cytokine that is characterized by several unique biological properties. IL-22 is a secreted cytokine produced mainly by cells of the lymphoid lineage and innate lymphoid cells. IL22 is a member of a group of cytokines called the IL-1 family of IL-1 superfamily a class of potent mediators of cellular inflammatory responses. IL22 has been shown to be an essential mediator in epithelial regeneration and wound repair. IL22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.IL22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-1R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1. IL22 also plays an important role in controlling the risk of inflammation-induced preterm birth.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse IL-22 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 22-35 kDa.

Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells. ED50 is 5-10 ng/mL.