| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -Ki67 Rabbit pAb | BKM-00582 | 100 μL |
Product Information
Protein full name | Proliferation marker protein Ki-67 |
Synonyms | MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human Ki67 |
Uniprot ID | P46013 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus |
Applications
IHC/IF | Human, Mouse, Rat | 1: 400-1: 1000 | colon cancer, esophageal cancer, intestinal cancer |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 500 | HeLa |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .
Images
| IHC analysis of KI67 (GB111499). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of KI67 (GB111499). Sample: Human esophageal cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of ki67 (GB111499). Sample: Mouse ovary (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of KI67 (GB111499). Sample: Rat small intestine (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IF analysis of ki67 (GB111499). Sample: Human esophagus (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 400, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of ki67 (GB111499). Sample: Human intestinal cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of ki67 (GB111499). Sample: Rat spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| ICC analysis of ki67 (GB111499). Sample: HeLa, 4% PFA (G1101) 20min. Antigen retrieval: 0.5% Triton X-100 (G1204), RT, 15 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of ki67 (GB111499). Sample: NIH3T3, 4% PFA (G1101) 20min. Antigen retrieval: 0.5% Triton X-100 (G1204), RT, 15 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of ki67 (GB111499). Sample: HeLa, 4% PFA (G1101) 20min. Antigen retrieval: 0.5% Triton X-100 (G1204), RT, 15 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: GB111499 ki67(green) 1:200,4℃ overnight; GB12200 alpha Tubulin(red) 1:400,4℃ overnight. Secondary antibody: Alexa Fluor 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 300 RT, 1h; Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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