Product Information

Applications

Special note

Epitope labeling has been proved to be an effective method that can be performed on target proteins. Tag antibody can be used in a variety of applications, including ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB and so on.

Background

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Images

	Western blot analysis of FLAG Tag (GB15938) at dilution of 1: 2000
	Immunocytochemistry analysis of 4% paraformaldehyde fixed transfected HEK-293T cells using FLAG Tag (GB15938) at dilution of 1: 600
	Immunoprecipitation of transfected HEK-293F cells lysate using Flag-Tag（IP: GB15938, 1: 100; Detection: GB11938, 1: 3000）

Storage

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.