| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Recombinant Anti-P53 antibody (Rabbit mAb) | BKM-05139 | 50 μL |
Product Information
Protein full name | Cellular tumor antigen p53 |
Synonyms | TP53, P53 |
Immunogen | Recombinant protein corresponding to Mouse p53 |
Uniprot ID | P04637,P02340,P10361 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus, Endoplasmic reticulum, Mitochondrion |
Predicted MW. / Observed MW. | 44 kDa / 53 kDa |
Applications
WB | Human | 1: 1000-1: 3000 | 293T,U-251 MG,T-47D |
IHC/IF | Human | 1: 500-1: 1000 | ovarian cancer |
Background
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases.
Images
| WB analysis of P53 (GB15627). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of P53(GB15627). Sample: Human esophageal carcinoma,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of P53(GB15627). Sample: Human ovarian cancer,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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