| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -Myelin Basic Protein Rabbit pAb | BKM-01273 | 100 μL |
Product Information
Description | Myelin Basic Protein rabbit polyclonal antibody |
Protein full name | Myelin Basic Protein |
Synonyms | MBP, entrez:4155, myelin basic protein |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Myelin Basic Protein |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Myelin membrane, Cytoplasmic side of myelin |
Predicted MW. | 27 kDa |
Observed MW. | 17/20 kDa |
Uniprot ID | P04370, P02688 |
Applications
WB | Mouse, Rat | 1: 500-1: 1000 | brain |
IHC/IF | Mouse, Rat | 1: 300-1: 600 | brain, striatum |
Background
The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function.
Images
|
|
WB analysis of Myelin Basic Protein (GB11226). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
|
|
IHC analysis of MBP (GB11226). Sample: Rat striatum (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
|
|
IF analysis of MBP (GB11226). Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of MBP (GB11226). Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
扫码关注 更多精彩
在线客服
