| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -Aggrecan Rabbit pAb | BKM-02554 | 100 μL |
Product Information
Protein full name | Aggrecan core protein |
Synonyms | ACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, Aggrecan |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Aggrecan |
Uniprot ID | Q61282 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Extracellular matrix |
Applications
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | bone |
Background
Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the lectican (chondroitin sulfate proteoglycan) family. This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
Images
| IHC analysis of Aggrecan(GB11373). Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:citrate buffer (H 6.0) (G1201)，98℃,20 min. Primary antibody:1: 500, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Aggrecan(GB11373). Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:citrate buffer (H 6.0) (G1201)，98℃,20 min. Primary antibody:1: 1000, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
 |
IHC analysis of Aggrecan (GB11373). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
 |
IHC analysis of Aggrecan (GB11373). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
 |
IHC analysis of Aggrecan (GB11373). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of Aggrecan (GB11373). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of Aggrecan (GB11373). Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IF analysis of Aggrecan (GB11373). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of Aggrecan (GB11373). Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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