| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti-Phospho-p53 (S15) Rabbit pAb | BKM-03685 | 100 μL |
Product Information
Protein full name | Cellular tumor antigen p53 |
Synonyms | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser15), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53, P-P53 |
Immunogen | KLH conjugated Synthetic phosphopeptide corresponding to Human p53（S15） |
Uniprot ID | P04637 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 44/38/39/34/30/24 kDa / 53 kDa |
Applications
WB | Human | 1: 300-1: 600 | HT29 |
Background
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.
Images
| Western blot analysis of p-p53 (S15) (GB115050) at dilution of 1: 300 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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