| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -SQSTM1 / p62 Rabbit pAb | BKM-03919 | 50 μL |
Product Information
Protein full name | Sequestosome-1 |
Synonyms | SQSTM1, A170, OSIL, PDB3, ZIP3, p60, p62, p62B, FTDALS3, Sequestosome 1, NADGP, DMRV |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human SQSTM1 |
Uniprot ID | Q13501 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | |
Predicted MW. / Observed MW. | 48 kDa / 62 kDa |
Applications
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, HIN3T3, HepG2 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | ovarian cancer, lung cancer |
ICC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, NIH/3T3 |
Background
The product of SQSTM1 gene, Sequestosome-1, also kown as ubiquitin-binding protein p62, is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. By interacting with GATA4 and targeting it for degradation, it can inhibit GATA-4 associated senescence and senescence-associated secretory phenotype.
Images
| Western blot analysis of P62 (GB11531) at dilution of 1: 500 Lane 1: HeLa (untreated) cell lysate Lane 2: HeLa (treated with Chloroquine, 50 μM, overnight ) cell lysate Lane 3: NIH3T3 (untreated) cell lysate Lane 4: NIH3T3 (treated with Chloroquine, 50 μM, overnight ) cell lysate Lane 5: HepG2 cell lysate Lane 6: MCF7 cell lysate Lane 7: A549 cell lysate Lane 8: L929 cell lysate Lane 9: PC-12 cell lysate |
 | Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using P62 (GB11531) at dilution of 1: 500 |
 | Immunohistochemistry analysis of paraffin-embedded human lung cancer using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed Hela cell untreatd using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed Hela cell with chloroquine treated using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cell untreatd using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cell with chloroquine treated using P62 (GB11531) at dilution of 1: 500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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