| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -beta Catenin Mouse mAb | BKM-04659 | 50 μL |
Product Information
Protein full name | Catenin beta-1 |
Synonyms | Beta-catenin, CTNNB1, CTNNB, OK/SW-cl.35, PRO2286, CHBCAT, CATNB, Cadherin associated protein |
Immunogen | Recombinant human beta-catenin protein expressed in E.coil |
Uniprot ID | P35222, Q02248, Q9WU82 |
Isotype | IgG1,κ |
Purity | Affinity purification |
Subcellular location | Cytoplasm, Cytoskeleton, Nucleus, Synapse, Cell projection, Cell membrane |
Predicted MW. / Observed MW. | 85 kDa / 92 kDa |
Applications
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | breast cancer, colon cancer, stomach, colon |
Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon.
Images
| WB analysis of β-catenin (GB12016). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of β-catenin (GB12016). Sample: Human breast cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of β-catenin (GB12016). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of β-catenin (GB12016). Sample: Mouse stomach (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of β-catenin (GB12016). Sample: Rat stomach (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of β-catenin (GB12016). Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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