| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -CD8 alpha Mouse mAb | BKM-04669 | 100 μL |
Product Information
Protein full name | T-cell surface glycoprotein CD8 alpha chain |
Synonyms | T-lymphocyte differentiation antigen T8/Leu-2, CD8a, MAL, Leu2, Leu2 T lymphocyte antigen, Ly 35, LYT3, p32, T8 T cell antigen |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human CD8 alpha |
Uniprot ID | P01732 |
Isotype | IgG1,κ |
Purity | Affinity purification |
Subcellular location | Cell membrane, Secreted |
Applications
IHC/IF | Human | 1: 1500-1: 3000 | colon cancer, colon, lung cancer |
Background
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Images
| IHC analysis of CD8A (GB12068). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of CD8A (GB12068). Sample: Human colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of CD8A (GB12068). Sample: Human lung cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of CD8A (GB12068). Sample: Human tonsil (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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