| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -beta III Tubulin Mouse mAb | BKM-04734 | 50 μL |
Product Information
Protein full name | Tubulin beta-3 chain |
Synonyms | TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin, TUJ-1,TUJ 1, TUJ1, tuj1 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse β3- tubulin |
Uniprot ID | Q9ERD7, Q4QRB4 |
Isotype | IgG2b,κ |
Purity | Affinity purification |
Subcellular location | Cytoplasm |
Predicted MW. / Observed MW. | 55 kDa / 55 kDa |
Applications
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1:1000-1:2000 | cerebral cortex, hippocampus, brain |
ICC/IF | Mouse | 1: 500-1: 1000 | Neuro-2a |
Background
Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.
Images
| Western blot analysis of β-III-Tubulin (GB12139) at dilution of 1: 1000 |
| Immunofluorescence analysis of mouse Neuro-2a cells using β3- tubulin (GB12139) at dilution of 1: 1000 A: DAPI nuclear stain ( blue ) B: Anti-β3- tubulin ( red ) C: Merge |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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