| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Anti -EGFP Mouse mAb | BKM-04835 | 100 μL |
Product Information
Protein full name | Enhanced GFP |
Synonyms | Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP |
Immunogen | Recombinant protein corresponding to EGFP(enhanced green fluorescent protein) |
Isotype | IgG1,κ |
Purity | Affinity purification |
Applications
WB | Species independent | 1: 500-1: 1000 | cells/ tissues with eGFP |
ICC/IF | Species independent | 1: 2000-1: 4000 | CHO-S |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.
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Images
| Western blot analysis of EGFP (GB12602) at dilution of 1: 1000 |
![]() | Immunocytochemistry analysis of paraffin embedded CHO-S using eGFP (GB12602) at dilution of 1: 1500 Blue: DAPI Red: Anti-eGFP (Cy3) |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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