| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Recombinant Anti-CD8 alpha Rabbit mAb | BKM-04998 | 100 μL |
Product Information
Protein full name | T-cell surface glycoprotein CD8 alpha chain |
Synonyms | CD8A, CD8, Leu2, MAL, p32,CD8a molecule, CD8 alpha |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse CD8 alpha |
Uniprot ID | P01731 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cell membrane |
Predicted MW. / Observed MW. | 26 kDa / 29 kDa |
Applications
WB | Mouse | 1: 500-1: 1000 | thymus, spleen |
IHC/IF | Mouse | 1: 400-1: 800 | 4T1 breast tumor model, colon, Lewis subcutaneous tumor model |
Background
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells.
Images
| WB analysis of CD8(GB15068). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of CD8(GB15068). Sample: Mouse 4T1 cell subcutaneous tumor model, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD8(GB15068). Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD8(GB15068). Sample: Mouse Lewis cell subcutaneous tumor model, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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