Product Information

Applications

Special note

Epitope labeling has been proved to be an effective method that can be performed on target proteins. Tag antibody can be used in a variety of applications, including ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB and so on.

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Images

	Western blot analysis of His Tag (GB151251)  at dilution of 1: 5000
	Immunoprecipitation of transfected HEK-293F cells lysate using His-Tag（IP: GB151251, 1: 100; Detection: GB151251, 1: 3000）

Storage

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.