| 产品名称 | 产品编号 | 规格 |
|---|---|---|
| Recombinant Anti-Cytokeratin 14 antibody (Mouse mAb) | BKM-05145 | 50 μL |
Product Information
Protein full name | Keratin, type I cytoskeletal 14 |
Synonyms | Krt14 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Cytokeratin 14 |
Uniprot ID | Q61781 |
Isotype | IgG1 |
Purity | Affinity purification |
Subcellular location | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 53 kDa / 53 kDa |
Applications
WB | Human | 1: 500-1: 1000 | A-431 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | esophageal carcinoma,skin |
Background
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Images
| WB analysis of CK14 (GB15803). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG(GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Cytokeratin 14(GB15803). Sample: esophageal carcinoma,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:1000, 4℃overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of Cytokeratin 14(GB15803). Sample: Mouseskin,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1:1000, 4℃overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of Cytokeratin 14(GB15803). Sample: Ratskin,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:1000, 4℃overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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