Gene Name Synonym

G-CSF, CSF3, Csfg, C17orf33, CSF3OS, MGC45931

Species

Mouse

Accession

P09920

Source

HEK293 Cells

SPECIFICATIONS

Amino acid sequence

Val31-Ala208 is expressed with a 10×His tag at the N terminus

Molecular Weight

20.4 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Granulocyte colony stimulating factor (G-CSF) is a cytokine with immunomodulation effects. Named for its relatively specific stimulation of the growth of neutrophil progenitor cells in vitro in semi-solid cultures G-CSF is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. G-CSF is a major extracellular regulator of haemopoiesis and the innate immune system. G-CSF influences the survival, proliferation and differentiation of all cells in the neutrophil lineage, from haemopoietic stem cell through to mature neutrophil. G-CSF, granulocyte-macrophage-colony stimulating factor (GM-CSF), and pegylated G-CSF are effective at reducing risk and duration of neutropenia-related events. The expression level of GCSF is significantly associated with the prognostic survival of Hepatocellular carcinoma (HCC), and it is expected to become a new prognostic marker of HCC, providing a novel idea for future basic research as well as targeted therapy.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse G-CSF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 50-150 pg/mL.