Gene Name Synonym

CSF-3, MGI-1G, GM-CSF, beta, MGC45931, Pluripoietin

Species

Human

Accession

P09919

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived Human G-CSF protein, Thr31-Pro207.

Molecular Weight

Approximately 19.0 kDa.

Purity

>97.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Granulocyte-colony stimulating factor(G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. G-CSF is a glycoprotein, secreted by the cells of the immune system, fibroblasts, and endothelium and functions to stimulate granulopoiesis, the innate immunity, and the differentiation of neural progenitor cells. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF conveys neuroprotection to central neurons upon increases in phosphorylation of PI3K/Akt pathway, and regulates epithelial to mesenchymal transition in cancer. G-CSF acts via a specific cognate receptor(G-CSFR) that belongs to the class I cytokine receptor superfamily. G-CSF is well known as a hematopoietic cytokine that stimulates the proliferation, differentiation, and function of myeloid progenitors and mobilization of hematopoietic stem and progenitor cells. G-CSF has the potential to inhibit the progression of atherosclerosis in animal models. G-CSF receptorwas expressed on cardiomyocytes and G-CSF activated the Jak/Stat pathway in cardiomyocytes.

Data

SDS-PAGE

Bioactivity

Recombinant Human G-CSF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 50-150 pg/mL.